Journal: Biochemistry and Biophysics Reports

Article Title: The immunosuppressive capacity of human mesenchymal stromal cells derived from amnion and bone marrow

doi: 10.1016/j.bbrep.2016.07.019

Figure Lengend Snippet: The characteristic of cultured MSCs. (A) BM-MSCs at day 7. (B) Oil red-O staining of BM-MSCs at day 21. (C) ALP staining of BM-MSCs at day 14. (D) AM-MSCs at day 10. (E) Oil red-O staining of AM-MSCs at day 28. (F) ALP staining of AM-MSCs at day 28. (G) Flow cytometric analysis of surface marker expression. The data shown are representative of the three different experiments.

Article Snippet: For adipogenic differentiation, 3×10 4 MSCs from each source were cultured in adipogenic differentiation medium (Hyclone ® Advance STEM™, USA) with complete change of medium every 3 days.

Techniques: Cell Culture, Staining, Marker, Expressing

Journal: Biochemistry and Biophysics Reports

Article Title: The immunosuppressive capacity of human mesenchymal stromal cells derived from amnion and bone marrow

doi: 10.1016/j.bbrep.2016.07.019

Figure Lengend Snippet: Mean value of proliferative index of responder T cells co-cultured with stimulator T cells or PHA activated T cells in the presence or absence of MSCs. #, * p <0.05 significantly different compared to MNC+stimulator and MNC+PHA, respectively.

Article Snippet: For adipogenic differentiation, 3×10 4 MSCs from each source were cultured in adipogenic differentiation medium (Hyclone ® Advance STEM™, USA) with complete change of medium every 3 days.

Techniques: Cell Culture

Journal: Biochemistry and Biophysics Reports

Article Title: The immunosuppressive capacity of human mesenchymal stromal cells derived from amnion and bone marrow

doi: 10.1016/j.bbrep.2016.07.019

Figure Lengend Snippet: (A–C) Relative gene expression in MSCs after cultured with PHA activated T cells or IFN-γ. (D, E) Western blot analysis of IDO expression in MSCs after cultured with PHA activated T cells or IFN-γ. * ,# p <0.05 significantly different compared to BM-MSCs+MNC and AM-MSC+MNC, respectively.

Article Snippet: For adipogenic differentiation, 3×10 4 MSCs from each source were cultured in adipogenic differentiation medium (Hyclone ® Advance STEM™, USA) with complete change of medium every 3 days.

Techniques: Expressing, Cell Culture, Western Blot

Journal: Biochemistry and Biophysics Reports

Article Title: The immunosuppressive capacity of human mesenchymal stromal cells derived from amnion and bone marrow

doi: 10.1016/j.bbrep.2016.07.019

Figure Lengend Snippet: Mean value of proliferative index of PHA activated T cells co-cultured with MSCs in the presence or absence of 1-MT (A), Indomethacin (B), and l -NAME (C). *, # , $p <0.05 significantly different compared to MNC+PHA+BM-MSCs, MNC+PHA+AM-MSC and MNC+PHA+BM-MSCs+1MT, respectively.

Article Snippet: For adipogenic differentiation, 3×10 4 MSCs from each source were cultured in adipogenic differentiation medium (Hyclone ® Advance STEM™, USA) with complete change of medium every 3 days.

Techniques: Cell Culture

Journal: Cell & Bioscience

Article Title: Characterization of a migrative subpopulation of adult human nasoseptal chondrocytes with progenitor cell features and their potential for in vivo cartilage regeneration strategies

doi: 10.1186/s13578-016-0078-6

Figure Lengend Snippet: The differentiation potential of mnCPC was compared to hCh and MSC and induced by growth factor supplemented differentiation media over a period of 21 (adipogenic and osteogenic differentiation) and 28 days (chondrogenic differentiation). Only MSC, cultured in adipogenic differentiation medium, exhibited fatty vacuoles positive with Oil red O staining ( a ). Strong calcium deposition—stained with von Kossa stain—was shown for MSC and mnCPC, only small deposits were found in hCh ( b ). In the pellet culture for chondrogenic differentiation, collagen type II was detected in all samples ( c ). All control groups, cultured in basal medium, were negative ( d ). The respective gene expression analysis ( e – g ) supported the findings visualised by histological and immunohistochemical staining. Graphs show individual values with median, n = 4–8 donors for each cell type, *p ≤ 0.05. Representative images were chosen

Article Snippet: The initial cell density was 3.15 × 10 4 cells cm −2 in MSC adipogenic differentiation medium (PromoCell, Heidelberg, Germany).

Techniques: Cell Culture, Staining, Expressing, Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: Characterization of 3D Organotypic Culture of Mouse Adipose-Derived Stem Cells

doi: 10.3390/ijms25073931

Figure Lengend Snippet: Assessment of 3D organotypic ADSC differentiation potential. ( A ) Representative images of 3D organotypic ADSCs during adipogenic differentiation on the 14th day. The decrease in organoid size relative to the nondifferentiation control group is apparent. Scale bar size is 200 μm. ( B ) Oil Red O staining performed on the 14th day of adipogenic differentiation. Positive staining for Oil Red O is observed only in peripheral cells of the matrix. Scale bar size is 400 μm. ( C ) Relative mRNA expression levels of adipocyte markers LPL and adipoQ in 3D ADSCs compared to 2D ADSCs as a positive control. The expression of LPL and adipoQ mRNA in 3D ADSCs was not detected, in contrast to their expression in 2D ADSCs. The values are the means ± standard deviations ( n = 4).

Article Snippet: Adipogenic induction media (STEMCELL Technologies, Vancouver, Canada) were then added and replaced every 3 days.

Techniques: Control, Staining, Expressing, Positive Control

Journal: Journal of Tissue Engineering

Article Title: Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders

doi: 10.1177/20417314211001545

Figure Lengend Snippet: Identification of Ad-MSCs (a–c) and AMEs (d–f): (a) characterization of the fourth generation of Ad-MSCs was performed by flow cytometry, and the Ad-MSC histogram revealed that after purification, the cells mostly expressed mesenchymal cell markers. Differentiation assays showed that Ad-MSCs could differentiate into adipocytes (b) and osteocytes (c), which were stained with Oil Red O and Von Kossa, respectively. Scale bar = 100 μm. (d) TEM of exosomes isolated from MSCM-cultured Ad-MSCs for 3 days with 10% exosome-depleted FBS. Scale bars = 100 nm. (e) NTA found that most of these vesicles ranged in diameter from 30 to 150 nm. (f) Western blotting was performed with Ad-MSCs (MSCs) and AMEs (Exos). The expression of CD9, CD63, TSG101, and calnexin was detected. Ad-MSCs: adipose-derived mesenchymal stem cells; AMEs: adipose-derived mesenchymal stem cell exosomes; NTA: nanoparticle tracking analysis; TEM: transmission electron microscopy.

Article Snippet: The medium was changed to mesenchymal stem cell adipogenic differentiation medium (MADM, ScienCell) and mesenchymal stem cell osteogenic differentiation medium (MODM, ScienCell) when the cells reached 70–80% confluency.

Techniques: Flow Cytometry, Purification, Staining, Isolation, Cell Culture, Western Blot, Expressing, Derivative Assay, Transmission Assay, Electron Microscopy